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Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates <t>MyHC</t> IIx–positive fibers, and green indicates <t>MyHC</t> <t>IIb–positive</t> fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.
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Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates <t>MyHC</t> IIx–positive fibers, and green indicates <t>MyHC</t> <t>IIb–positive</t> fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.
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Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates <t>MyHC</t> IIx–positive fibers, and green indicates <t>MyHC</t> <t>IIb–positive</t> fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.
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Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates <t>MyHC</t> IIx–positive fibers, and green indicates <t>MyHC</t> <t>IIb–positive</t> fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.
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Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates MyHC IIx–positive fibers, and green indicates MyHC IIb–positive fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.

Journal: PLOS One

Article Title: Alaska pollock protein as a functional dietary source for promoting skeletal muscle hypertrophy and lipid metabolic remodeling

doi: 10.1371/journal.pone.0348366

Figure Lengend Snippet: Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates MyHC IIx–positive fibers, and green indicates MyHC IIb–positive fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.

Article Snippet: Sections were fixed with acetone and incubated overnight at 4°C with primary antibodies against MyHC IIa (SC-71) and MyHC IIb (BF-F3), obtained from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA).

Techniques: Immunofluorescence, Staining